مشخصات پژوهش

صفحه نخست /Evaluation of miR-141, ...
عنوان
Evaluation of miR-141, miR-200c, miR-30b Expressionand Clinicopathological Features of Bladder Cancer
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Urinary bladder neoplasm, microRNA-200c, microRNA-141, microRNA-30b, neoplasm grading, neoplasm invasiveness
چکیده
Bladder cancer (BC) ranks the second most common genitourinary tract malignant tumor with high mortality and 70% recurrence rate worldwide. MiRNAs expression has noticeable role in bladder tumorigenesis. The purpose of this study was to assess miR-200c, miR-30b and miR-141 in tissue samples of patients with BC and healthy adjacent tissue samples and their association with muscle invasion, grade and the size of the tumor. Transurethral resection tissue samples were collected from thirty- five newly diagnosed untreated patients with BC from 2013 to 2014. The control group consisted of adjacent normal urothelium. All samples, observed by two pathologists, were diagnosed transitional cell carcinomas (TCC) with the proportion of tumor cells greater than 80%.Total RNA including miRNAs was extracted from about 50 mgtissue samples by applying TRIzol reagent. 2 (-∆∆CT) method was used to calculate relative quantificationof miRNA expression. Two of 35 patients were females and the other 33 were males. Invasion to bladder musclewas observed in 13 (37%) cases. MiR-141, miR-200-cand miR30-b were up-regulated in 91%, 79% and 64% of malignant tissues, respectively. Down-regulation of miR-141 had a strong association with muscle invasion (P= 0.017). Significant inverse correlation between grading and miRNA-141 level was observed (P= 0.043).
پژوهشگران علی مهدوی نژاد (نفر اول)، سید حبیب ا... موسوی بهار (نفر دوم)، جلال پورالعجل (نفر سوم)، رضا یادگار آذری (نفر چهارم)، محمد جعفری (نفر پنجم)، مسعود سعیدی جم (نفر ششم به بعد)