مشخصات پژوهش

صفحه نخست /Dissemination of ...
عنوان
Dissemination of extended-spectrum beta lactamases (ESBLs) and qnr genes among clinical isolates of uropathogenic Escherichia coli in children
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Escherichia coli (E.coli), ESBLs, UTI, Quinolones, Antibiotic resistance
چکیده
Background: Urinary tract infection (UTI) is one of the most common childhood bacterial infections and E.coli is the major pathogen. Producing Betalactamase enzymes are the most common mechanism of bacterial resistance. Objective: The aim of this study was to determine the prevalence of ESBLs and qnr genes in E.coli strains isolated from UTIs. Method: In this study, a total of 120 isolates of E.colifrom urinary tract infections of the children, which were collected at BESAT hospital in Hamadan, west of Iran,from October 2010 to October 2011, included. The isolates diagnosis was done by standard biochemical methods. Antimicrobial susceptibilities were determined by disk diffusion method, ESBLs- producing was confirmed phenotypically using the double-disk synergy (DDS) test.The presence and identity of ESBLs and qnrgenes were determined by polymerase chain reaction (PCR). Results: The highest sensitivity was seen respectively to Imipenem (96.7%),Amikacin (92.5%),Nitrofurantoin (93.3%), Ofloxacin (81.7%), Gentamicin and Norfloxacin (70.8%) and Ciprofloxacin (79.2%); in contrast the highest rate of resistance was seen respectively for Cotrimoxazole (77%) and NalidixicAcid (40.9%).The results showed that 6(2.18%) and 4(1.12%) E.coli-producing isolates of ESBL were positive in case of existing qnrB and qnrS genes respectively. qnrA was not diagnosed in any isolates. Conclusion: CTX-Mwas the most prevalent ESBL genotype in uropathognicE. Coli(UPEC) isolated from UTI. In addition, a high frequency of qnr genes among ESBL- producing E. coliwas identified in this study.It is recommended that in order to avoid treatment failures, use phenotypic and molecular methods for experiments related to diagnose these enzymes and qnr genes.
پژوهشگران ایرج صدیقی (نفر اول)، محمدرضا عربستانی (نفر دوم)، زهرا کریمی تبار (نفر چهارم)، محمد یوسف علیخانی (نفر پنجم)