مشخصات پژوهش

صفحه نخست /Genetic Identification of ...
عنوان
Genetic Identification of Trichomonas vaginalis by Using the Actin Gene and Molecular Based Methods
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Actin gene, SSCP, Trichomonas vaginalis
چکیده
Background: Trichomonas vaginalis is the agent of urogenital tract infection that causes human trichomoniasis with some serious health complications. More under-standing about genetic features of the parasite can be helpful in the study of the pathogenesis, drug susceptibility and epidemiology of the infection. For this end we conducted analysis of the actin gene of T. vaginalis by applying the PCR-SSCP (PCR-Single Stranded Conformational Polymorphism) and nucleotide sequencing method. Methods: Fifty T. vaginalis samples were collected from 950 women attending gy-necology clinics in two cities of Iran, Hamadan and Tehran, from November 2010 to July 2011. After axenisation of isolates, all samples subjected to PCR-SSCP and nucleotide sequencing. Results: According to the SSCP banding patterns and nucleotide sequencing, sev-en sequence types were detected among the isolates. Alignment of the nucleotide sequences showed five polymorphic sites in the different strain types. Amino acid substitution was not observed in the nucleotide sequence translation of the all se-quences Conclusion: The actin gene analysis represents genetic diversity of T. vaginalis and it suggests that various strains can be responsible for clinically different trichomo-niasis in infected individuals. It is expected that further studies will be conducted to increase our knowledge about relationship between the actin gene polymor-phism and different biological behavior of the parasite..
پژوهشگران محمد متینی (نفر اول)، صغری ربیعی (نفر ششم به بعد)