مشخصات پژوهش

صفحه نخست /Association of G22A and ...
عنوان
Association of G22A and A4223C Adenosine deaminase-1 (ADA1) gene polymorphisms and ADA activity with polycystic ovary syndrome (PCOS)
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Adenosine Deaminase Alleles Genotypes Polycystic Ovary Syndrome Single Nucleotide Polymorphism
چکیده
Adenosine deaminase-1 (ADA1) regulates concentration of adenosine as main modulator of oocyte maturation. There are compelling evidences for the association of ADA1 gene polymorphisms with many diseases but the importance of ADA1 polymorphisms in polycystic ovary syndrome (PCOS) has not been studied before. This study investigates serum total ADA activity (tADA), ADA1 and ADA2 isoenzyme activities, and genotype and allele frequencies of G22A and A4223C polymorphisms in healthy and PCOS women. In this case-control study 200 PCOS patients and 200 healthy women were enrolled. Genomic DNA was extracted from whole blood and PCR-RFLP technique was used to determine the G22A and A4223C variants. The genotype frequencies were calculated and the association between polymorphic genotypes and enzyme activities were determined. tADA activity was significantly lower in PCOS compared with control (27.76±6.0 vs 39.63±7.48, respectively). PCOS patients also showed reduced activity of ADA1 and ADA2. PCOS was not associated with G22A polymorphism whereas AA, AC, and CC genotypes of A4223C polymorphism were found distributed differently between control and PCOS women where C allele showed a strong protection role for PCOS (Odd ratio=1.876, p=0.033). The present study for the first time showed that lower ADA activity may involve in pathogenesis of PCOS through leaving higher concentration of adenosine in environment and affecting follicular growth. As a novel finding, we also showed great differences in genotype distribution and allele frequencies of A4223C polymorphism between groups indicating a protection role for C allele against PCOS.
پژوهشگران مهشید صالح آبادی (نفر اول)، مرضیه سنوئی فریمانی (نفر دوم)، حیدر طیبی نیا (نفر سوم)، مرضیه قربانی (نفر چهارم)، فرانک پورمنصفی (نفر پنجم)، جلال پورالعجل (نفر ششم به بعد)، غلامرضا شفیعی (نفر ششم به بعد)، ندا قاسمخانی (نفر ششم به بعد)، ایرج خدادادی کهلان (نفر ششم به بعد)