مشخصات پژوهش

صفحه نخست /Comparison of Resistant and ...
عنوان
Comparison of Resistant and Susceptible Strains of Trichomons vaginalis to Metronidazole Using PCR Method
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Trichomonas vaginalis , Metronidazole, Resistance, PCR
چکیده
Backgr ound: Metronidazole is drug of choice recommended by WHO for treatment of trichomoniasis, however, some reports claims drug resistance in Trichomonas vaginalis isolates recently. The objective of this study was to determine the minimum lethal concentration (MLC) of metron i- dazole in resistant and sensitive strains, as well as genetic patterns of these stains by PCR method. Methods : From February 2006 to March 2007, in a cross sectional study, clinical and wet mount e x- amination of vaginal smear along with cu lture were performed on 683 women attending to public and private outpatient clinics in Hamadan. Trichomoniasis marked based on major clinical symptoms. Diagnosis confirmed using wet mount microscopically and culture in Diamond medium. A serial concentrati on of metronidazole was provided and all isolated Trichomonas strains (resistant and sens i- tive) tested by standard method. Finally, all sensitive and resistant strains examined by PCR tec h- nique. Results : Only 15/683, (2.2%) of patients clinically diagnosed trichomonal vaginitis were positive for T. vaginalis by wet smear and culture. The minimum lethal concentration (MLC) for clinically sens i- tive isolates was 25 μg/ml; however, this concentration for resistant isolates was 200 μg/ml after 24 h and 100 μg/ml after 50 h. The results of PCR examination of DNA from sensitive and resistant isolates had same pattern. The lanes appeared by two primers were 98 bp and 261 bp for both clin i- cally sensitive and resistant strains. Conclusion : Resistance to metronidazole in T. vaginalis has not relation to genetic variations and might be related to some physiologic pathways of organism.
پژوهشگران صغری ربیعی (نفر اول)، محمد متینی (نفر سوم)، محمد فلاح (نفر چهارم)