مشخصات پژوهش

صفحه نخست /Hepatoprotective effect of ...
عنوان
Hepatoprotective effect of parthenolide in rat model of nonalcoholic fatty liver disease
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Cytochrome P450 CYP2E1 High-fat diet NF-kappa B Non-alcoholic fatty liver disease Parthenolide Tumor necrosis factor-alpha
چکیده
Abstract Context: The active ingredients of traditional medical herbs have been the focus of scientific interests. Objective: This study was designed to explore the mechanisms of actions of parthenolide on nonalcoholic fatty liver disease (NAFLD). Materials and methods: Thirty five male Wistar rats were fed high-fat diet (HFD) for eight weeks with or without an intraperitoneal injection of parthenolide to develop NAFLD. Liver triacylglycerol (TG), total antioxidant capacity (TAC), total oxidative status (TOS), thiobarbituric acid reactant substances (TBARs), total thiol groups, and tumor necrosis factor alpha (TNF-α) and cytochrome P4502E1 (CYP2E1) levels as well as liver ALT, AST, and catalase activities were determined. In addition quantitative real-time PCR was performed to obtain hepatic gene expression levels of TNF-α, CYP2E1, and nuclear factor-κB (NF-κB). Results: HFD caused a significant weight gain and increased liver TG content as well as alteration in ALT and AST activities which all were attenuated after administration of parthenoide (p<0.05). Weakened liver antioxidant system (TAC, total thiol groups, and catalase activity) and increased oxidative stress markers (TBARs and TOS) were mainly ameliorated by parthenolide treatment (p<0.05). Increased hepatic TNF-α, NF-κB, and CYP2E1 at the both gene expression and protein levels were found associated with necroinflammatory changes in histopathological observations and were abrogated almost completely after parthenolide treatment. Oxidative and inflammatory changes observed in HFD fed rats were indicative of NAFLD which were suppressed with parthenolide treatment. Conclusions: Based on these results, parthenolide might be a candidate agent for preventing NAFLD due to its anti-inflammatory and anti-oxidative potency.
پژوهشگران مجید بهابادی (نفر اول)، عادل محمدعلی پور (نفر دوم)، جمشید کریمی (نفر سوم)، نسرین شیخ (نفر چهارم)، قاسم سلگی (نفر پنجم)، فرجام گودرزی (نفر ششم به بعد)، محمد هاشم نیا (نفر ششم به بعد)، ایرج خدادادی کهلان (نفر ششم به بعد)