مشخصات پژوهش

صفحه نخست /Ameliorative Effects of ...
عنوان
Ameliorative Effects of Nilotinib on CCl4 Induced Liver Fibrosis Via Attenuation of RAGE/HMGB1 Gene Expression and Oxidative Stress in Rat
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
HMGB1 Protein; Liver Cirrhosis; Nitric Oxide; Rats
چکیده
Nilotinib as a tyrosine kinaseinhibitor has been recently used to improve the liver fib-rosis process, but the exact mechanisms still require further clarification. In this study, we investigated the anti-fibrotic effects of Nilotinib via RAGE/HMGB1axis and anti-oxidant mechanisms. This experimental study was performed in the Hamadan University of Medical Sciences, Iran, from May 2015 to December 2016. Liver fibrosis was induced in Wistar male rats by CCL4. Rats were gavaged daily with Nilotinib (10 mg/kg). RAGE, HMGB1, TNF-and TGF-mRNA expression were evaluated by quantitative RT-PCR. TNF-protein levels were measured using the immunoassay method. Thiol groups, carbonyl groups, nitricoxide levels and glutathione peroxidase activity were measured by spectrophotometric methods.The results showed that Nilotinib decreased TNF-, TGF-, RAGE and HMGB1 mRNA expression (p<0.001) in the liver tissues of the fibrosis group. Nilotinib also decreased carbonyl groups and nitric oxide levels and increased thiol groups and glutathione peroxidase activity in the fibrosis groups. The histopathological changes were found to be attenuated by Nilotinib. In conclusion, Nilotinib can improve liver fibrosis and open new mechanisms of the anti-fibrotic properties of Nilotinib
پژوهشگران جمشید کریمی (نفر دوم)، ایرج خدادادی کهلان (نفر سوم)، قاسم سلگی (نفر ششم به بعد)