مشخصات پژوهش

صفحه نخست /Hydrogen peroxide: A potent ...
عنوان
Hydrogen peroxide: A potent inducer of differentiation of human adipose-derived stem cells into chondrocytes
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Adipose tissue; Chondrogenesis; Differentiation; Hydrogen peroxide; Mesenchymal stromal cells
چکیده
Abstract Common protocols for chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) are generally expensive and time-consuming and so far, have not successfully recreated pure chondrocytes. We hypothesize that a low level of H2O2 may induce differentiation of ADSCs into chondrocytes in a shorter incubation time and relatively lower cost. Therefore, this study aimed to comparatively investigate the effectiveness of H2O2 containing or free medium in the induction of ADSCs to chondrocytes. ADSCs were isolated from the lipoaspirate of four healthy females and evaluated by immunophenotyping for their CD90, CD73, CD44, CD34, and CD45 cell surface markers. Chondrogenic differentiation was carried out using differentiation medium in the presence or absence of 10 and 50 µM H2O2 in normal and 3D culture system. The intracellular contents of reactive oxygen species (ROS) were detected by flow cytometry and fluorescence microscopy. The hydroxyproline, was assessed as marker of collagen and the glycosaminoglycans (GAGs) content were both qualitatively detected and quantitatively determined. Real-time PCR was performed to determine the gene expression level of aggrecan, type-II collagen, and transcription factor Sox9. H2O2 treated cells showed pre-chondrocyte morphology on day 1 and chondrocyte pellets were formed on day 14. H2O2 treated cells induced greater pellet sizes and showed significantly higher content of GAGs and hydroxyproline level compared with untreated cells. The gene expression levels of aggrecan, collagen type II, and Sox9 were markedly upregulated by H2O2. Our findings showed for the first time that H2O2-containing differentiation medium is potentially more effective than H2O2 free differentiation medium in the induction of chondrogensis of ADSCs.
پژوهشگران فرجام گودرزی (نفر اول)، عادل محمدعلی پور (نفر دوم)، مجید بهابادی (نفر سوم)، محمد تقی گودرزی( بازنشسته) (نفر چهارم)، آرش سرو آزاد (نفر پنجم)، ایرج خدادادی کهلان (نفر ششم به بعد)