مشخصات پژوهش

صفحه نخست /Silymarin prevents apoptosis ...
عنوان
Silymarin prevents apoptosis through inhibiting the Bax/caspase-3 expression and suppresses toll like receptor-4 pathway in the SNc of 6-OHDA intoxicated rats
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Silymarin Apoptosis Oxidative stress Neurodegeneration TLR Parkinsonʼs disease
چکیده
RRECTED Background and purpose: Several lines of evidence show that apoptosis, oxidative stress and neuroinflmmation are associated with the development of Parkinsonʼs disease (PD). In the present study, we investigated the effect of pre-treatment with silymarin (SM) on oxidative stress, apoptosis and toll-like receptor 4 (TLR4) expression in substantia nigra pars copmacta (SNc) of 6-hydroxydopamine (6-OHDA)-lesioned rats. Methods: Animals were pretreated with 100, 200 or 300 mg/kg of SM daily for 5 days and at 6th day 6-OHDA (8 μg/2 μl) was infused unilaterally into the central region of the SNc. Results: 6-OHDA decreased the total glutathione and antioxidant enzymes activity in the SNc. Interestingly, we found that 6-OHDA caused to TLR4 up regulation. The SNc levels of glutathione, superoxide dismutase, glu tathione peroxidase, glutathione reductase and catalase were significantly higher in the SM pretreated rats. SM strongly decreased 6-OHDA-induced elevation of SNc apoptosis, caspase-3 and Bax/Bcl-2 ratio. Furthermore, SM markedly (p < 0.001) prevented from SNc over expression of TLR4 caused by 6-OHDA. A significantly high pos itive correlation was seen between TLR4 activity with caspase-3 protein levels (r= 0.896, P < 0.01), Bax protein levels (r= 0.96, P < 0.01). Conclusion: Pre-treatment of 6-OHDA-lesioned rats with SM reduces SNc neuronal apoptosis possibly through in hibition of TLR4 over expression. Further clinical study should be carried out to prove potential application of SM for protection against PD in susceptible individuals.
پژوهشگران رسول حدادی (نفر اول)