چکیده
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Purpose ADAMTS-1 and 9 play a crucial role in the ovulation and their altered levels may play a role in the pathogenesis
of polycystic ovary syndrome (PCOS). The aim of this study was to assess ADAMTS-1 and 9 expression and their correlation with the oocyte quality and maturity in the cumulus cells (CCs) of PCOS patients and normovulatory women during
an IVF procedure.
Methods Expression of ADAMTS-1 and 9 and progesterone receptors (PRs) in the CCs containing MII and germinal vesicle
(GV) oocytes of 37 PCOS patients and 37 women with normal ovulatory function who underwent IVF treatment was evaluated using qRT-PCR. Moreover, correlation between ADAMTS-1 and 9 expression and oocyte quality were also investigated.
Results mRNA expression levels of ADAMTS-1 and ADAMTS-9 were signifcantly reduced in the women with PCOS
compared to the normovulatory women. ADAMTS-1 and ADAMTS-9 mRNA expression levels in the CCs showed a considerable correlation. Lower expression levels of ADAMTS-1 and ADAMTS-9 in PCOS patients were strongly correlated
with diminished oocyte maturation. There was a remarkable association between ADAMTS-1 and ADAMTS-9 mRNA
expression levels and oocyte quality. PRs (PRA and PRB) were dramatically decreased in PCOS patients when compared
with the control group.
Conclusions The results of the present study indicated that ADAMTS-1 and ADAMTS-9 as well as PRs are downregulated
in the human CCs in PCOS patients, which could be associated with impaired oocyte maturation and may result in a lower
oocyte recovery and oocyte maturity rates, as well as lower fertilization rate.
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