مشخصات پژوهش

صفحه نخست /Molecular Typing of Brucella ...
عنوان
Molecular Typing of Brucella Species Isolated from Humans and Animals Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Technique
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Omp2, Pst1, PCR, Brucella,
چکیده
Background: Brucellosis is a zoonotic disease that causes major economic and public health problems. It is one of the most im-portant diseases in humans and domestic animals. Hence, the exact identification of Brucella spp. is important for strategies of treatment and control. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is one of the molecular techniques characterized by amplification of deoxyribonucleic acid (DNA) sequence and restriction enzyme digestion. Objectives: This study aimed at identifying genetic polymorphisms of omp2a genes among 90 Brucella isolated from humans and animals, using the PCR-RFLP method. Methods: Ninety Brucella spp. isolated from humans and animals in two different regions of Iran were used in this study. Bio-chemical tests and the Brucella omp2a (1100bp) gene-PCRwas used for identificationof Brucella isolates. Polymerase ChainReaction productsweredigestedby restrictionendonuclease enzyme pstI andgene sequencinganalysiswas carriedout formolecular typing of Brucella strains. Therefore, genetic relatedness was revealed by a dendrogram. Results: Analysisof the90 Brucella strainsbybiochemical tests, PCR, andPCR-RFLPmethodswith PstI enzymeand omp2a sequencing showed four unique RFLP Profiles (P1-P4). Seventy-nine (87.8%) of the Brucella isolates belonged to B. melitensis strain 20236. From30 animal isolates, nine (30%) belonged to B. melitensis biovare1 and two (6.6%) to B. abortus strain. According to the RFLP dendrogram, group 1 and 2 hadhigher genetic relatedness similarity. Conclusions: The results showed B. melitensis strain20236was the predominant strainamonghumanandanimal Brucella isolates. Likewise, according todendrogramresults, thePCR-RFLP techniquewasnot able toseparatehumanandanimal speciesof B.meliten-sis from B. abortus
پژوهشگران نسرین بهمنی (نفر اول)، سیدحمید هاشمی (نفر دوم)، محمدرضا عربستانی (نفر سوم)، فریبا کرامت (نفر ششم به بعد)، محمد یوسف علیخانی (نفر ششم به بعد)