مشخصات پژوهش

صفحه نخست /Antibacterial effect of ...
عنوان
Antibacterial effect of eucalyptus (globulus Labill) and garlic (Allium sativum) extracts on oral Cariogenic bacteria
نوع پژوهش مقاله چاپ شده
کلیدواژه‌ها
Plant extracts, Cariogenic agents, Dental caries, Streptococcus mutans, Lactobacillus
چکیده
The antibacterial activity of the plant extracts including garlic and eucalyptus extracts against cariogenic bacteria, Streptococcus mutans and Lactobacillus acidophilus were investigated. To evaluate the effect of different concentrations of alcoholic extracts of eucalyptus and garlic on S. mutans and Lactobacillus acidophilus, 72 sheep blood Muller Hinton agar were prepared. The sensitivity profile of the bacteria was determined according to the method of Bauer-Kirby. The sterile blank papers stained by seven different concentrations of the extracts were dried and put on the media inoculated with the test organisms. Four media from each group were considered as controls, two of which were added and the other two were added effective antibiotics, penicillin (10 IU) and erythromycin (15 μg/ml) disks. Inhibition zones were measured after 24 h of incubation at 37°C in the presence of 5% CO2. Interpretation of resistance was compared with the media containing effective antibiotics. Both bacteria, S. mutans and Lactobacillus acidophilus, were resistant to eucalyptus extract but sensitive to garlic extract. The inhibition zone diameter increased as concentration of garlic extract increased. Furthermore, both bacteria were susceptible to penicillin and erythromycin. However, S. mutans was more sensitive to erythromycin while Lactobacillus acidophilus was more sensitive to penicillin. It seems that garlic extract has a potential antibacterial effect against cariogenic agents such as S. mutans and Lactobacillus acidophilus in vitro, while eucalyptus extract had no effect. However, we need more scientific evidence based on randomized clinical trials in order to investigate the antimicrobial activity of garlic against these bacteria in vivo and its effect on prevention or reduction of dental caries.
پژوهشگران جلال پورالعجل (نفر چهارم)